Double Haploid Lab
Double Haploid Lab
The Double Haploid Lab is located in Fairbanks, ÀÖ»¢Ö±²¥ in the Arctic Health Research Building at the ÀÖ»¢Ö±²¥.
This lab uses tissue culture techniques to rapidly develop genetically uniform wheat, oats, barley and canola lines. Traditional breeding requires four to five years of repeated selection to achieve this level of genetic uniformity. Using double haploidy (DH) technology, the lab can produce comparable pure lines in about 300 days, greatly accelerating crop breeding and variety development. Researchers are developing new varieties of wheat, oats and barley to grow in ÀÖ»¢Ö±²¥; studying which traits are required for crops to thrive in ÀÖ»¢Ö±²¥â€™s changing climate and growing season; and identifying the genes that control those traits.
History
This lab was founded in 2025 with five years of funding support from a USDA National Institute of Food and Agriculture grant.
Facilities
The lab facilities include:
- Thermo Scientific Heraguard ECO Lean Bench
- Incubators (for different temp)
- Filtration units / vacuum pump
- Labomed LX400 Phase Photo port Compound Microscope
- Luxeo 6Z Stereo Microscope with DF/BF/LED Goose neck Base and camera port head
- DeNovix DS-II Spectrophotometer
- Thermo Scientific OWL D2-BP
- Fisherbrand AccuSpin 17R is a refrigerated benchtop centrifuge
- Fisherbrand Electrophoresis Power Supply
- Mastercycler X40 (PCR machine)
- Thermo Scientific Basic Vortex Mixer
- Refrigerators (4 °C) for short-term reagents and cultures
- Freezers (–20 °C) for medium-term storage
- Ultra-low freezer (–80 °C) for long-term storage of cultures and DNA/RNA
Currently, this lab does not provide any services to outside entities. In the near future, we plan to offer DH line production services on a cost-recovery basis to other institutions and plant breeding programs.
